Anti-Mullerian Hormone (AMH) ELISA Kit
Basic Information
Congenital bilateral non-testosterone with AMH secretion and no testosterone secretion is characterized by a male pseudohermaphroditism. There are no female Müller-derived gonadal organs and no Wolff-derived gonadal organs if they are in the process of embryo development. Tissue secretion of testosterone, then the male Golff-derived gonadal organs can develop small to a certain extent, reflecting that embryonic development is a testosterone-dependent developmental stagnation. If untreated congenital bilateral non-testis patients develop puberty Will be stagnant and present a typical type of testicular-free appearance because unilateral has been able to meet physiological functional needs, so unilateral congenital testosterone patients will not have adolescent gender dysplasia, but there may be unilateral Müller tube residual, of course, depending on The time the organization lost.
Anti-Mullerian Hormone (AMH) ELISA Kit
Color and color
Color development
Color development is the last incubation step in an ELISA assay where the enzyme catalyzes a colorless substrate to produce a colored product. The temperature and time of the reaction are still factors that affect color development. Properly increasing the temperature helps to accelerate color development. The negative holes remain colorless for a certain period of time, while the positive holes are colored for a prolonged period of time. In the quantitative test, the reaction temperature and time after the addition of the substrate should be determined according to the regulations. The ELISA coloration result is best carried out by a microplate reader, which can reduce the experimental error and improve the analysis accuracy of the critical value sample. Try to avoid direct judgment of the results by the naked eye, because the visual differences of different individuals. It should be noted that when the developer is added, the developer is kept out of the flow. Avoid adding more bubbles when adding stop solution, otherwise the probability of false positive results may increase.
Colorimetric
The colorimetric method includes two methods: a visual method and an enzyme standard colorimetric method. The visual method is simple and straightforward, but for the same specimen, the operator's difference sometimes has different results and has certain subjectivity. Colorimetric results are usually expressed in terms of optical density and are now expressed as absorbance as specified. The microplate reader should not be placed under sunlight or strong light. The suitable room temperature during operation is between 15 °C and 30 °C. Preheat the instrument for 15~30 minutes before use, so that the measured results are more accurate. Before colorimetry, clean the liquid attached to the bottom of the plate with a clean absorbent paper, and then place the plate correctly on the colorimetric frame of the enzyme-based colorimeter. In summary, high-quality reagents, good instrumentation and correct operation are necessary conditions to ensure accurate and reliable ELISA results. In the actual application process, the operator must be proficient in basic operational skills, conduct strict and careful verification of each link, try to avoid the occurrence of false positive and false negative reactions, ensure the test results are true and reliable, and provide reliable diagnosis for clinical diagnosis and disease prevention. The theoretical basis.
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