Column DNA gel recovery kit operating instructions - Huaqiang Electronic Network

Products and Features

This kit is designed for the efficient recovery of DNA fragments from agarose gels. It provides a professional and reliable solution for molecular biology experiments.

The kit utilizes a DNA-binding silicone membrane combined with a unique sol system, allowing for the recovery of DNA fragments ranging from 50 bp to 40 Kb. It works effectively with TAE, TBE, and SuperBuffer gels, offering a recovery efficiency of up to 90%.

Key Features of the Kit:

1. High Efficiency: Achieves up to 90% recovery rate for DNA fragments.

2. Fast Process: The entire procedure can be completed in just 10 minutes.

3. High Purity: The recovered DNA is suitable for various downstream applications such as digestion, ligation, PCR, and more.

4. Versatile Application: Can recover single-stranded, double-stranded, and circular DNA.

5. Cost-effective: More affordable compared to most similar products on the market.

6. Easy Storage: The kit can be stored at room temperature for several months without any loss of performance.

Usage and Results

To use the kit, add 3 times the volume (approximately 0.3–0.5 mL) of universal sol solution to the agarose gel containing the DNA fragment or the DNA reaction mixture. Incubate the mixture at 65°C for 5–10 minutes. Transfer the solution into the centrifuge column and spin for about 1 minute. Then, add 500–600 μL of universal wash solution and centrifuge for 30 seconds. Repeat the washing step once. After that, spin for 30 seconds to remove any remaining liquid. Place the spin column into a new tube, add 30–50 μL of DNA elution buffer, and centrifuge for 30 seconds to collect the purified DNA.

Frequently Asked Questions

Q: Why is it not recommended to recover DNA from TBE gels using a silica membrane column?

A: The boric acid present in TBE gels can interfere with the DNA binding process by reacting with the hydroxyl groups on the surface of the silica membrane. These hydroxyl groups are essential for DNA adsorption. For a detailed explanation, please refer to the technical documentation titled "Silica-DNA Binding Principle and Influencing Factors."