Column DNA gel recovery kit operating instructions - Huaqiang Electronic Network

Products and Features

This is a DNA recovery kit designed specifically for isolating DNA fragments from agarose gels. It offers a fast, efficient, and professional method to recover DNA with high purity and reliability.

The kit uses a DNA-binding silicone membrane combined with a unique sol system, allowing the recovery of DNA fragments ranging from 50 bp to 40 Kb from TAE, TBE, and SuperBuffer gels with a recovery efficiency of up to 90%. The process is simple and quick, making it ideal for various molecular biology applications.

Key Features of This Product:

• High efficiency: Up to 90% recovery rate of DNA fragments.
• Fast procedure: The entire process takes only about ten minutes.
• High purity: Recovered DNA is suitable for downstream experiments such as digestion, ligation, PCR, and more.
• Versatile use: Works well with single-stranded, double-stranded, and circular DNA.
• Cost-effective: More affordable compared to similar products in the market.
• Easy storage: Can be stored at room temperature for several months without loss of performance.

Usage and Effect

To use the kit, add 3 times the volume (approximately 0.3–0.5 mL) of universal sol solution to the agarose gel containing the DNA fragment or to the DNA reaction solution. Incubate the mixture at 65°C for 5–10 minutes. Transfer the solution into a centrifuge spin column and centrifuge for about 1 minute. Then, add 500–600 μL of universal wash solution and centrifuge for 30 seconds. Repeat the washing step once. Afterward, centrifuge again to remove any remaining liquid. Place the spin column into a new tube, add 30–50 μL of DNA elution buffer, and centrifuge for 30 seconds to collect the purified DNA.

FAQ

Q: Why is it not recommended to recover DNA from TBE gels using a silica gel membrane column?

A: TBE contains boric acid, which can react with the hydroxyl groups (-OH) on the surface of the silica gel. These hydroxyl groups are essential for the binding of DNA to the silica membrane. This reaction can reduce the efficiency of DNA recovery. For a detailed explanation, please refer to the technical data sheet titled "Silica-DNA Binding Principle and Influencing Factors."