How to reduce the error in the ELISA kit experiment? -Huaqiang Electronic Network

EL-C1600N100013-B
Test - lowercase jpg
Kaixin micro test
Test probe P100-M3

Elisa kit

It is an in vitro ELISA for detection of anti-human hepatitis E (HEV) virus IgM antibody in human serum or plasma.

The ELISA kit test is a highly sensitive, specific, and reproducible experimental diagnostic method. Because of its stable reagents, easy storage, easy operation, and objective judgment, it has been widely used in various fields of immunology.


but do you know? If you make the following small mistakes during the experiment, there will be experimental errors!


1. Speak less when doing experiments to prevent saliva from falling into the enzyme label.


2. Adding a primary antibody secondary antibody requires 37°.


3. If you do multiple boards at the same time, multiple boards should be together; use as few shots as possible.


4. When loading, add from low concentration to high concentration, thus reducing the chance of jumping.


5. Change the gun head frequently.


First, the use of pipettes, sample (antibody) and other reagents that need accurate quantification do not use a row of guns, experience has proved that the gun is very inaccurate, easy to jump holes, do not buy cheap tips, because the ELISA will not only be amplified The target protein also amplifies non-specifically bound impurity proteins.


2. When diluting the sample, the dilution factor is preferably less than 10.


3. All containers containing reagents related to ELISA kits, glass products to be dried or disposable resin containers.



It is derived from the advanced enzyme-linked immunosorbent technology Shanghai Jinma Experimental Equipment Co., Ltd., covering the experience and wisdom of domestic and foreign experts. The use of advanced technology and equipment to ensure quality while reducing costs, for more researchers in need, save experimental funds, ensure the quality of experiments, and contribute to the country's scientific and technological development. A number of bio-research base partners, the strength team is dedicated to creating a focus on

ELISA kit

Production, research and development, and help your research experiments

!

More developments in science and technology. A number of bio-research base partners, the strength team is dedicated to creating a focus on

ELISA kit

Production, research and development, and help your research experiments

!


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