Comparison of Yaxin Recombinant Aprotinin (RTI16) and Sigma Extract Aprotinin (A1153)
EXPERIMENTAL METHOD:
1. Determination of protein content, specific activity, absorbance, and acidity test: A known amount of powder from both enzymes was weighed and dissolved in pure water to prepare a 2.5g/ml enzyme solution. Protein content was determined using the absorption coefficient method at 280 nm, where 1 mg/ml corresponds to an absorbance of 0.84. Activity was measured according to the standard operating procedure (SOP), and specific activity was calculated. The enzyme solution was diluted to 3 EPU/ml, and the absorbance was measured at 277 nm. The acidity of the solution was also tested and should not exceed 0.8.
2. Determination of HPLC Purity:
- Column: TSK-GEL G2000SWXL (molecular sieve analysis column) - pH range: 2.5–7.5 - Flow rate: 0.5–1.0 ml/min (up to 1.2) - Temperature: 10–30°C - Eluent salt concentration: less than 0.5M - Detection wavelength: 280 nm
- Method: The following conditions were used:
Method | pH | Flow rate (ml/min) | Temperature (°C) | Eluent | Molecular Sieve |
5.8 | 1 | 25 | 0.1M HAC-NaAC, pH 5.8, 0.1 M NaCl | TSK-GEL G2000SWXL |
- Other methods followed the liquid chromatography SOP. - Sample load: 20 µg - Sample preparation: Dissolved in pure water from lyophilized powder.
EXPERIMENTAL RESULTS:
1. Protein Content, Specific Activity, Absorbance, and Acidity:
Protein Content (%) | Specific Activity (EPU/mg) | Absorbance | Acidity (pH) | |
Yaxin RTI | 72% | 3.71 | 0.505 | 5.0–7.0 |
Sigma Aprotinin | 76.9% | 3.56 | 0.526 | 5.0–7.0 |
2. HPLC Purity:
- Yaxin Recombinant Aprotinin (RTI161101):


- Sigma Aprotinin (Cat. No. A1153, Lot No.: SLBP2656V):


CONCLUSION: The results showed that the protein content, specific activity, absorbance, and acidity of both proteins are very similar. However, the specific activity of Yaxin Recombinant Aprotinin (RTI161101) was slightly higher than that of Sigma Aprotinin (A1153). In terms of HPLC purity, both samples exhibited 100% purity, with Yaxin’s sample showing a more concentrated peak, indicating better separation and consistency in the chromatographic profile.
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