Heme oxygenase 1 (HO-1) elisa kit - Database & Sql Blog Articles

Heme Oxygenase 1 (HO-1) ELISA Kit

Introduction to Basic Information

Heme Oxygenase (HO) is the rate-limiting enzyme in heme degradation, catalyzing the conversion of heme into biliverdin IX, carbon monoxide (CO), and iron. Biliverdin is subsequently reduced to bilirubin. Three HO isoforms have been identified: HO-1, HO-2, and HO-3. HO-2 and HO-3 are constitutively expressed in many tissues and may function as heme-binding proteins under normal conditions. In contrast, HO-1 is an inducible enzyme that can be activated by various stressors such as oxidative stress, heat shock, UV radiation, ischemia-reperfusion injury, heavy metals, bacterial lipopolysaccharides, cytokines, nitric oxide (NO), and its substrate heme.

Recent studies have highlighted the biological significance of the products of the HO reaction, including antioxidant, anti-inflammatory, anti-apoptotic, signal transduction, immunomodulatory, and anti-adhesion molecule activities. The strong induction of HO-1 in response to various stimuli suggests it plays a crucial role in protecting against inflammation and oxidative tissue damage. This makes HO-1 an important target in research related to disease mechanisms and therapeutic strategies.

Heme Oxygenase 1 (HO-1) ELISA Kit

Introduction to Identification Methods

Quantitative Determination

The ELISA procedure involves multiple steps and is sensitive to numerous variables, especially during the coating of the solid-phase carrier. To ensure accurate results, each test batch should include a series of reference standards with known concentrations. A standard curve is then constructed under identical experimental conditions. The ELISA kit used for this purpose typically employs a sandwich format, suitable for detecting large molecular weight substances. The standard curve usually spans a wide range, with the maximum absorbance value often approaching 2.0. The curve is plotted on semi-logarithmic paper, where the concentration of the analyte is on the x-axis and the absorbance is on the y-axis. The resulting curve is typically S-shaped, with the steepest portion representing the most reliable detection range.

Qualitative Determination

Qualitative determination provides a simple yes/no or positive/negative result based on the presence or absence of a detectable signal in the sample. While the result is primarily qualitative, it can also yield semi-quantitative data through titer analysis. This involves serial dilutions of the sample, with the highest dilution showing a positive response indicating the titer. This method offers more precise insights compared to undiluted samples and helps assess the strength of the reaction in a more quantitative manner.

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